DeNovoGUI Crack+ Activator [Win/Mac] [April-2022] Thai-box jellyfish venom extraction and purification A poster was presented at the 7th International Allergens Symposium. Thai-box jellyfish are venomous animals that have large stingers (Figure 1). They are found in the tropical and subtropical regions and are distributed worldwide. The venomous products of these jellyfish can cause various severe injuries in humans, including death. According to the World Health Organization, over a period of 5 years, the number of reported stings reached 2.5 million (in the countries where the jellyfish are found), although more cases of stings are likely to be unreported. Recently, some Taiwanese hospitals reported treating over 500 patients suffering from Thai-box jellyfish sting injuries, with a total hospitalization time of nearly 2 years. This study was to investigate the venom-extracting system and the venom purification process for the jellyfish, and to identify the proteins in the venom. Venom extractions from 10 jellyfish tentacles, five tentacles, and five jellyfish ovaries were carried out, respectively. Two venom extraction processes, using water and methanol as the solvents, were performed to extract the venom, respectively. The crude venom was then subjected to 2-DE and gel digestion. In-gel trypsin digestion and LC-MS/MS analysis were carried out to identify the venom proteins. Eleven venom proteins were identified, including 2 metalloproteases, one serine protease, one disintegrin, one cysteine-rich protein, 1 venom allergen and 6 other venom proteins. This study revealed the venom-extracting system and purification process for the jellyfish. Moreover, 11 venom proteins were identified, which might be important in the development of the therapy and prevention of injuries. A poster was presented at the 7th International Allergens Symposium. Thai-box jellyfish are venomous animals that have large stingers (Figure 1). 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